GDF9下调转基因小鼠模型对繁殖与生长的影响

doi:10.11843/j.issn.0366-6964.2015.07.011

Abstract

Abstract:

This experiment aims to detect the effect of down-regulation of GDF9 on reproduction and growth in GDF9 shRNA transgenic mice through statistical analysis of the litter size and weight gain of transgenic mice and detection of biomolecular and blood biochemical indexes such as GDF9 shRNA，GDF9 and serum FSH and GH level after reproduction.The levels of GDF9 shRNA and GDF9 mRNA expression were detected in hypothalamus，pituitary and ovary via RT-PCR，and serum FSH and GH level were examined using ELISA.The results showed that 16 GDF9 shRNA transgenic mice obtained were positive，and GDF9 shRNA in transgenic mice was widely expressed in various tissues.Litter sizes between transgenic and wild mice were significantly different (P<0.05)；Body weight of transgenic male mice was higher than that of control mice with significant difference (P<0.05) in the seventh week，and body weight of female mice did not show significant difference.Compared with the negative mouse，GDF9 gene expression in transgenic mice in hypothalamus and pituitary were decreased by 35.4% and 39.8% (P<0.05)，respectively.In transgenic female mice，GDF9 expression in ovaries was not significantly decreased (P>0.05)，but its serum FSH level was 55% higher than the control (P<0.01).The present study showed down-regulation expression of GDF9 gene can improve the ability of animal reproduction.It may provide a favorable material and molecular target for producing transgenic large livestock with high reproduction.

CLC Number:

S814.8

XI Qian-yun，JIAO Li，XIAO Min，ZHANG Yong-liang. The Effect of Transgenic Mice Model Down-regulated GDF9 on Reproduction and Growth[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1157-1162.

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The Effect of Transgenic Mice Model Down-regulated GDF9 on Reproduction and Growth

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